Journal: Oncotarget

Article Title: An innovative diagnostic strategy for the detection of rare molecular targets to select cancer patients for tumor-agnostic treatments

doi: 10.18632/oncotarget.27343

Figure Lengend Snippet: * Tissue categories are reported in the management software of the pathology department. At fifteen-days/1 month intervals (depending on the number of samples available) the recorded information are used for sample selection and construction of TMA and TSA, with the tissues arranged by organ pathology (See Material and Methods). ** In the case of neoplastic forms for which routine clinical molecular testing is planned (i.e non-small cell lung cancer, colorectal carcinoma, melanoma etc.), TMA and TSA construction must be performed after the analysis of conventional predictive tests. Abbreviations: IHC, immunohistochemistry; FISH, fluorescence in situ hybridization; RT-PCR, real-time PCR; NGS, next generation sequencing

Article Snippet: From 50 up to 150 cancer cells per patient were scored, and signals were evaluated using the FISH imaging and capturing software SoloTouch (Bioview Duet; BioView, Rehovat, Israel).

Techniques: Software, Selection, Immunohistochemistry, Fluorescence, In Situ Hybridization, Reverse Transcription Polymerase Chain Reaction, Real-time Polymerase Chain Reaction, Next-Generation Sequencing